Biofilm Management In Poultry

Biofilm Management In Poultry

Water is essential in controlling body temperature, supports the immune system, assist in food digestion, and aids in the processing and elimination of body wastes. Regardless of procurement of water, another concern is contamination of water inside the watering systems through a substance called “BIOFILM. It occurs when bacteria attach to the pipe walls in a drinking system, creating a sticky substrate.

Biofilms are commonly defined as attached bacterial colonies of either single or multiple species, encased in an extracellular matrix.

Biofilms support the survival of bacteria in suboptimal conditions and increase resistance to disinfectants, antimicrobial, and antibiotics. To date, it is estimated that 99% of bacteria can grow in biofilms, and it is suggested that for the majority of bacteria, biofilms are the normal mode of existence.

Plant essential oils like Thyme, Eucalyptol, Clove, Citronella, etc. Have been used for hundreds of years as natural medicines to combat a multitude of pathogens, including bacteria, fungi, and viruses. Several essential oils confer antimicrobial activity by damaging the cell wall and membrane, leading to cell lysis, leakage of cell contents, and inhibition of proton motive force.  In addition, there is evidence that they effectively kill bacteria without promoting the acquisition of resistance.

A blend of all such essential oils is HERBOFLOXIN”-This micro emulsified system;

  • The target zone for Herbofloxin is the gut; it has an antimicrobial effect in the gut due to which there is no biofilm formation.
  • It improves the number of good bacteria in the gut and simultaneously reduces the pathogenic bacteria. This action of Herbofloxin in turn provides a natural growth promoter effect in Poultry.
  • Herbofloxin is freely soluble in water and target species include Salmonella, E. Coli, Campylobacter and Clostridium

5 Tips To Combat Bio Aerosols In Poultry

Combat Bio Aerosols In PoultryAerial pollutants in confined animal houses are widely recognized as detrimental to the respiratory health of animals kept in these facilities. Primary and opportunistic microbial pathogens may directly cause infectious and allergic diseases in farm animals, and chronic exposure to some types of aerial pollutants may exacerbate multi-factorial environmental diseases. The key pollutants recognized in the airspace of livestock buildings are particles, including dust, micro-organisms and their toxins, and gases such as ammonia, carbon dioxide and more than 100 trace gases such as volatile fatty acids. Under commercial production conditions the airborne particles will contain a mixture of biological material from a range of sources, with bacteria, toxins, gases and volatile organic compounds adsorbed to them. Hence, a more descriptive term for these airborne particles is bio-aerosol. Their sizes can range from aerodynamic diameters of 0.5 to 100 µm.

How are bio-aerosols formed?

Under commercial production the airborne particles will contain a mixture of biological material from a range of sources. The chickens produce large amounts of dust as a result of epithelial desquamation, as well as from feed, manure, faeces and litter (Matković et al., 2009). This dust consists of a variety of airborne particles of biological origin, i.e. Bacteria, fungi, endotoxins (lipopolysaccharide, LPS) of Gram-negative bacteria, 1.3-beta-glucan of fungi, fungal spores and mycelium fragments. Hence, a more descriptive term for these airborne particles is bio-aerosol in which the microorganisms can occur either as liquid droplets or as dry particles.

To prevent this problem in poultry, Vinayak Ingredients has introduced a very effective product called “ESSENTIOLITT POULTRY”. Essentiolitt Poultry is a blend of essential oils which works as a poultry sanitizer and reduces the formation of Bio-aerosols. Its features are as below:

  1. 50% reduced respiratory diseases.
  2. It posses potential as natural agents for airborne bacterial activity inhibitors.
  3. They are ecological products for insect pest control.
  4. Reduction in mortality of birds due to severe respiratory problems.
  5. It is acting as a Bio-security in Farms.

Dimension of Water for Poultry Birds

Dimension of Water for Poultry Birds - Vinayak IngredientsWater management is the most critical components in a top-performing broiler flock. In high performing flocks, at around 21˚C, cutting edge broilers by and large will consume 1.8 to 2 times more water than feed, in weight. Water consumption will change, contingent upon ecological temperature, feed quality and bird health:

  1. Water consumption increases by 6% for each expansion in 1˚C between 20-32°C.
  2. Water consumption increases by 5% for each expansion in 1˚C between 32-38°C.
  3. Feed consumption diminishes by 1.23% for each expansion in 1˚C over 20°C.

Any considerable change in water utilization should be researched as this may show a water leak, health issues or feed problem. A drop in water consumption is frequently the primary marker of a flock issue. To assess flock performance legitimately we have to know how much water birds are consuming each day.

Large portions of today’s high performance broilers are being brought up in housing built for the broiler of the past. There are numerous alternatives used to guarantee a water volume/stream will address bird issues at high pinnacle request times. Advanced water meters associated with the house controller can screen water consumption on a 24-hour premise as well as in distributed time increases amid the day. This data can figure out whether our water system is keeping up at basic ‘appeal’ times, as when the lights go ahead after a dull period. Knowing this can be extremely useful in finding performance issues on a ranch, particularly one with huge, multi-houses.

The temperature of the water that birds are drinking should also be considered. The perfect water temperature ought to associate with 10-14˚C originating from the source. Water consumed by the birds should not be over 30˚C. In the event that this happens the drinking system ought to be flushed intermittently to look after cooler, fresher water.

Along with temperature and accessibility the quality of water offered to chickens should be considered for proper growth of Broilers. The quality of water depends on several factors such as the presence of bacteria and other microbes, PH, Level of minerals etc. Disinfectants like Chlorine are widely used in the poultry industry for disinfection of water, but it has many disadvantages such as carcinogenicity, pH dependent activity, highly corrosive and irritating substance. To avoid such problems and to improve performance of broilers Vinayak ingredients has launched an excellent product Herbofloxin which is a blend of essential oils effective as Non- antibiotic growth promoter. It is used to replace disinfectants, antibiotics and acidifier used for growth promotion in water.

MOA of Herbofloxin: – It is a micro emulsion when mixed with a water forms nanoemulsion making it easier to penetrate bacterial cell wall and disrupt it. It is stable at 5 to 45 degree Celsius temperature and has long shelf life.

The advantages of Herbofloxin are as follows:-

  • More than 10% improvement in weight gain
  • Chlorine free meat
  • Pathogen free meat
  • No antibiotic residue
  • Carcass sanitizer
  • Litter improver

Micro Emulsions in Poultry

Micro Emulsions in PoultryMicro emulsions in Poultry are dispersion’s of oil and water with an emulsifier. They are clear, thermodynamically stable, isotropic liquid mixtures. They are super solvents which improves stability and thermodynamic activity of formulation. Micro emulsions are beneficial to be used because it increases the efficacy of the formulation allowing dose reduction. The average particle size of micro emulsion is 0.1 micrometers which helps in increasing the inter facial area, thereby allowing active ingredients to get released easily. In poultry, micro emulsions are designed to include natural essential oils cell wall which in turn binds to mycotoxins to protect animals against mycotoxosis.

Vinayak Ingredients have introduced micro emulsion which is an alternative to antibioticsnamed as Herbofloxin. It is of natural origin prepared from essential oil of syzygium, citronella, thymus, eucalyptus. Herbofloxin has a particle size less than 0.1 micrometer which makes it easily soluble in water. It maintains poultry gut’s pH-6.5 to 6.7 which is slightly acidic. As it is a micro emulsion it has better dispersion in water, stable at 45 degree Celsius temperature and has a longer shelf life. All these factors make it safe to be consumed by poultry without having any side effects which are otherwise usually caused by using antibiotics. Herbofloxin is the natural replacer for antibiotic growth promoters.

Mechanism of action: Herbofloxin being a micro emulsion when mixed with water forms nano emulsion due to which particle size decreases further, making it easier to penetrate the bacterial cell wall and disrupt it. Disruption of cell wall leads to killing of bad bacteria such as Escherichia coli, Campylobacter, Clostridium and Salmonella. Thus, it acts like a bacteriostatic. It also acts as an anti-inflammatory by improving mucin coverage, which is a first line of defense in poultry.

Herbofloxin as a micro emulsion replaces antibiotics such as tetracycline’s, fluoroquinolones, amino glycosides and selectively modulates poultry gut to promote the beneficial microflora.

Role of Antioxidants in Feed

Role of Antioxidants in FeedAntioxidants in feed play a major role in animal health, production and performance. This is due to the detrimental effects of radicals and toxic products of their metabolism on various metabolic processes. It is a well known fact that oxidative stress is involved in many degenerative disorders. The oxidative free radicals are therefore considered as pathobiochemicals mechanism for initiating or progression of various diseases. The prooxidant-antioxidant balance can be regulated by optimal nutrient uptake or providing herbal antibiotics. Thus, the essential step in maintaining the balance between the oxidative damage and antioxidative defence in the animal body would be to boost the antioxidant capacity by optimising the dietary intake of antioxidants.

Vitamin C is a water-soluble antioxidant. It is an important anti-stress agent. However, it can be easily oxidized. Sources of vitamin C are citrus fruits and vegetables. Vitamin C is required in collagen biosynthesis and protein metabolism.

Vitamin E is the found in the biological membranes and lipid droplets. Vitamin E is absorbed in the small intestine with various efficacious depending on the diet composition, level of supplementation, age, sex and other individual characteristics of animals. It is the main chain-breaking antioxidant in biological systems.

Carotenoid is a natural pigment, responsible for yellow, orange and sometimes red pigmentation’s in plants, insects, birds and marine animals. They possess antioxidant activity. They have some health promoting properties, including immune system modulation. They are found in some plant-derived feed ingredients.

Manganese has an essential part of a range of enzymes taking part in antioxidant protection, bone growth and egg shell formation carbohydrate and lipid metabolism including processing of cholesterol.

Zinc is the second most abundant trace element trace element in mammals and they take part in antioxidant defence as an integral part of SOD, hormone secretion, keratin generation and epithelial tissue integrity immune function.

Iron has a vital role in antioxidant defence as an essential component of catalase, energy and protein metabolism, hence respiratory carrier, electron transport, oxidation-reduction reaction.

Changing Trends in Antibiotic Use in the Poultry Industry

Changing Trends in Antibiotic Use in the Poultry IndustryA major feature of poultry production nowadays is the reduction of in use of antibiotics as growth promoters, and this is majorly due to concerns over bacterial resistance.

Antibiotics used in a widespread manner as a feed additive can lead to development of Bacterial resistance as the residual antibiotics in the tissues of poultry can be consumed by humans. The bacteria resistant to antimicrobials may also get transferred from one individual to another individual leading to reduced performance of antimicrobials in humans.  This prompted the WHO in 1997 and the Economic and Social Committee of the European Union in 1998 to conclude that the use of antimicrobials in poultry could be a possible risk for the general health and well being of humans.

Many organisations are focusing their research on the development of alternative strategies to maintain the gut health of poultry and enhance performance of poultry by using various other substances, commonly known as natural growth promoters (NGPs). These Natural Growth Promoters have been identified as effective alternatives to antibiotics. A good popularity has been gained by Phytobiotics as NGPs especially, due to their beneficial effect on gut health, performance of birds and positive effects on the immunity of the bird.

Phytobiotics can be defined as plant derived products added to feed in order to improve performance. They originate from leaves, roots, tubers or fruits of herbs, spices and other plants. Phytobiotics are basically of plant origin, and popular extracts that are gaining interest among researchers and poultry producers are thyme, oregano, turmeric and garlic.

Keeping in mind the changing trends in the market, Vinayak Ingredients launched a natural growth promoter by the brand name Herbofloxin. Herbofloxin maintains the health of the gut and as an alternative to antibiotics. It has phyto-constituents of antibacterial nature that target the gut pathogens including important zoonotic bacteria. As a result, Herbofloxin promotes the growth of commensals.

Herbofloxin provides immune-modulator effect which improves tolerance to pathogenic bacteria and improves gastrointestinal micro-climate so as to improve the digestibility and uptake of nutrients. All these results almost nullify the requirement of antibiotics making it as an effective natural antimicrobial growth promoter.

Herbofloxin-Natural Antibiotic Growth Promoter

Herbofloxin-Natural Antibiotic Growth Promoter - Vinayak Ingredients

Herbofloxin is our answer to the long standing problem of antibiotic inclusions in feed and water of livestock. It is a unique micro emulsified formulation of phytoactive ingredients working as antibacterial, antiviral and antifungal solutions for monogastric organisms.

Herbofloxin eliminates chemicals such as, water sanitizers, pH regulators, feed disinfectants and antibiotics from Livestock production. It improves gut immune barriers to resist colonization by pathological microflora. Herbofloxin is a complete solution for providing a barrier against infective agents and safeguards the entry of zoonotic organisms in the food chain. Herbofloxin reduces gut colonization by E.coli, Salmonella and Campylobacter and thus reducing the zoonosis of the same in the meat. It is a growth promoter resulting in healthy weight gain.

Antibiotics!!! Do we need them?

Antibiotics Do the livestock need them - Vinayak Ingredients

Ever wondered why antibiotics are good, bad or evil? Well if you need an orientation session with the use and misuse of antibiotics, this is your space to read.

We all know definitions are important, so here we go. For a technically sound person the word ‘Antibiotics’ are agents which work against microorganisms. To put it into lay man perspective, these are substances/compounds which kill or slow down the growth of harmful bacteria, fungi or similar bugs that thrive on other living or dead things.  By the end of that “Layman term” explanation we would have understood that there isn’t a layman term for antibiotics and you don’t have to Einstein or for that matter Sheldon your way (Courtesy big bang theory) to understand the science behind antibiotics.

To make things clearer lets classify them into four practical categories (Oh I would love to classify them into seven different ways so that you need to classify the classification types to complicate matters, but this not what we intend to graduate in).

  • Chemically – Molecular structures (Beta lactams, Quinones, Aminoglycosides……)
  • Target microorganisms (Antibacterial, Anti-fungal, Antiprotozoal… )
  • Mechanism of action(Cell wall, enzyme, DNA….)
  • Evolutionary ( 1st generation, 2nd gen, New world )

Use of Antibiotics in the feed industry

Firstly not all antibiotics those are used as feed additives for animals and used in humans for treatment. The importance of this fact is rivaling many issues on banning of antibiotics as feed additives.  We will touch base with this statement again when we deal with the statement of antibiotic resistance. In the livestock industry antibiotics are used primarily for three reasons

  1. Treatment of bacterial, fungal and other infectious diseases.
  2. Prevention of infections , as a precautionary in feed compulsion.
  3. Growth promotion,to improve final body weight of birds raised for meat.

Many antibiotics used in treatment of livestock used for meat purpose are the same antibiotics used in humans. These include a major share of quinolones. Although the figures below in the table reflect otherwise one should also consider that these antibiotics given under the treatment regime are given for a small 4 or 5 day schedule and often used in very small quantities. If proper withdrawal periods are used these antibiotics are excreted from the body of the animal well before slaughter. The table below shows us the volume compartmentalisation of antibiotic use.

Use by volume Humans Animal
Pencilins 44 % 6 %
Cephalasporins 15% 1%
Sulfa 14% 3%
Quinolones 9% less than 1%
Macrolides 5% 4%
Tetracyclines 4% 41%
Ionophores 0% 30%

In the next blog we learn more about antibiotic resistance and how it impacts us …